Making standards for quantitative real-time pneumococcal PCR
نویسندگان
چکیده
منابع مشابه
Making standards for quantitative real-time pneumococcal PCR
Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantifica...
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Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...
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ژورنال
عنوان ژورنال: Biomolecular Detection and Quantification
سال: 2014
ISSN: 2214-7535
DOI: 10.1016/j.bdq.2014.11.003